Publications

Chhipi-Shrestha JK., Yoshida M.*, and Iwasaki S.*:
“Filter trapping protocol to detect aggregated proteins in human cell lines”
STAR Protoc. 3(3):101571. (2022)
Chen M., Kumakura N. (equal contribution), Muller R., Shichino Y., Nishimoto M., Mito M., Gan P., Ingolia NT., Shirasu K., Ito T., and Iwasaki S.*:
“A parasitic fungus employs mutated eIF4A to survive on rocaglate-synthesizing Aglaia plants”
BioRxiv. (2022)
Matsuura-Suzuki E., Shimazu T. (equal contribution)*, Takahashi M., Kotoshiba K., Suzuki T., Kashiwagi K., Sohtome Y., Akakabe M., Sodeoka M., Dohmae N., Ito T.*, Shinkai Y.*, and Iwasaki S.*:
“METTL18-mediated histidine methylation on RPL3 modulates translation elongation for proteostasis maintenance”
eLife. 11:e72780. (2022)
BioRxiv. (2021)
Press Release (RIKEN)
Yamada A., Toya H., Tanahashi M., Kurihara M., Mito M., Iwasaki S., Kurosaka S., Takumi T., Fox A., Kawamura Y., Miura K., and Nakagawa S.:
“Species-specific formation of paraspeckles in intestinal epithelium revealed by characterization of NEAT1 in naked mole-rat”
RNA. rna.079135.122. (2022)
RNA Journal Twitter (2022)
BioRxiv. (2022)
Shichino Y.* and Iwasaki S.*:
“Compounds for selective translational inhibition”
Curr Opin Chem Biol. 69:102158. (2022)
Fang Q., Kimura Y., Shimazu T., Suzuki T., Dohmae N., Iwasaki S., and Shinkai Y.:
“HEMK1 methylates glutamine residue of the GGQ motif of mitochondrial release factors”
Sci Rep. 12(1):4104. (2022)
Research Square. (2021)
Kimura Y., Saito H. (equal contribution), Osaki T., Ikegami Y., Wakigawa T., Ikeuchi Y., and Iwasaki S.*:
“Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation”
RNA. 28(6):895-904. (2022)
RNA Journal Twitter (2022)
BioRxiv. (2022)
Apostolopoulos A. and Iwasaki S.*:
“Into the matrix: current methods for mitochondrial translation studies”
J Biochem. 171(4):379-387. (2022)
Wu Q., Shichino Y., Abe T., Suetsugu T., Omori A., Kiyonari H., Iwasaki S., and Matsuzaki F.:
“Selective translation of epigenetic modifiers affects the temporal pattern and differentiation of neural stem cells”
Nat Commun. 13(1):470. (2022)
BioRxiv. (2020)
Mishima Y., Han P., Ishibashi K., Kimura S., and Iwasaki S.:
“Ribosome slowdown triggers codon-mediated mRNA decay independently of ribosome quality control”
EMBO J. 41(5):e109256. (2022)
BioRxiv. (2021)
Press Release (RIKEN)
Press Release (Kyoto Sangyo University)
Research Results (AMED)
Fujita T., Yokoyama T., Shirouzu M., Taguchi H., Ito T., and Iwasaki S.*:
“The landscape of translational stall sites in bacteria revealed by monosome and disome profiling”
RNA. 28(3):290-302 (2022)
RNA Journal Twitter (2022)
Kashiwag K., Shichino Y. (equal contribution), Osaki T. (equal contribution), Sakamoto A., Nishimoto M., Takahashi M., Mito M., Weber F., Ikeuchi Y., Iwasaki S.*, and Ito T.:
“eIF2B-capturing viral protein NSs suppresses the integrated stress response”
Nat Commun. 12(1):7102. (2021)
BioRxiv. (2021)
Press Release (RIKEN)
Press Release (The University of Tokyo)
Research Results (AMED)
Chadani Y., Sugata N., Niwa T., Ito Y., Iwasaki S., and Taguchi H.:
“Nascent polypeptide within the exit tunnel stabilizes the ribosome to counteract risky translation”
EMBO J. 40(23):e108299. (2021)
BioRxiv. (2021)
Press Release (RIKEN)
Press Release (Tokyo Institute of Technology)
Kumakura N., Singkaravanit-Ogawa S., Gan P., Tsushima A., Ishihama N., Watanabe S., Seo M., Iwasaki S., Narusaka M., Narusaka Y., Takano Y, and Shirasu K.:
“Guanosine-specific single-stranded ribonuclease effectors of a phytopathogenic fungus potentiate host immune responses”
BioRxiv. (2021)
Shichino Y., Mito M., Kashiwagi K., Takahashi M., Ito T., Ingolia NT., and Iwasaki S.*:
“A specific eIF4A paralog facilitates LARP1-mediated translation repression during mTORC1 inhibition”
BioRxiv. (2021)
Chhipi-Shrestha JK., Schneider-Poetsch T., Suzuki T., Mito M., Kha K., Dohmae N., Iwasaki S.*, and Yoshida M.:
“Splicing modulators elicit global translational repression by condensate-prone proteins translated from introns”
Cell Chem Biol. 29(2):259-275.e10. (2022)
BioRxiv. (2020)
Press Release (RIKEN)
Press Release (The University of Tokyo)
Press Release (Nikkei)
Ichihara K., Matsumoto A., Nishida H., Kito Y., Shimizu H., Shichino Y., Iwasaki S., Imami K., Ishihama Y., and Nakayama KI.:
“Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons”
Nucleic Acids Res. 49(13):7298-7317. (2021)
Press Release (RIKEN)
Press Release (Kyushu University)
Iwakawa HO., Lam Y.W.A., Mine A., Fujita T., Kiyokawa K., Yoshikawa M., Takeda A., Iwasaki S., and Tomari Y.:
“Ribosome stalling caused by the Argonaute-miRNA-SGS3 complex regulates production of secondary siRNA biogenesis in plants”
Cell Rep. 35(13):109300. (2021)
BioRxiv. (2020)
Press Release (RIKEN)
Press Release (The University of Tokyo)
Makino S., Kawamata T., Iwasaki S.*, and Ohsumi Y.:
“Selectivity of mRNA degradation by autophagy in yeast”
Nat Commun. 12(1):2316. (2021)
Press Release (RIKEN)
Press Release (Tokyo Institute of Technology)
Editors’ Highlights
Recommended in Faculty Opinions
Yoshimoto R., Chhipi-Shrestha JK., Schneider-Poetsch T., Furuno M., Burroughs MA., Noma S., Suzuki H., Hayashizaki Y., Mayeda A., Nakagawa S., Kaida D., Iwasaki S.*, and Yoshida M.:
“Spliceostatin A interaction with SF3B1 limits U1 snRNP availability and causes premature cleavage and polyadenylation”
Cell Chem Biol. 28(9):1356-1365.e4. (2021)
Press Release
Higashi H., Kato Y., Fujita T., Iwasaki S., Nakamura M., Nishimura Y., Takenaka M., and Shikanai T.:
“The pentatricopeptide repeat protein PGR3 is required for the translation of petL and ndhG by binding their 5'UTRs”
Plant Cell Physiol. 62(7):1146–1155. (2021)
Chen M., Asanuma M., Takahashi M., Shichino Y., Mito M., Fujiwara K., Saito H., Floor SN., Ingolia NT., Sodeoka M., Dodo K., Ito T., and Iwasaki S.*:
“Dual targeting of DDX3 and eIF4A by the translation inhibitor rocaglamide A”
Cell Chem Biol. 28(4):475-486.e8. (2021)
Press Release
Journal Cover
Preview
Mito M., Mishima Y., and Iwasaki S.*:
“Protocol for disome profiling to survey ribosome collision in humans and zebrafish”
STAR Protoc. 1(3):100168. (2020)
Matsuki Y., Matsuo y., Nakano Y., Iwasaki S., Yoko H., Udagawa T., Li S., Saeki Y., Yoshihisa T., Tanaka K., Ingolia NT., and Inada T.:
“Ribosomal protein S7 ubiquitination during ER stress in yeast is associated with selective mRNA translation and stress outcome”
Sci Rep. 110(1):19669. (2020)
BioRxiv. (2020)
Suzuki Ta., Yashiro Y., Kikuchi I., Ishigami Y., Saito H., Matsuzawa I., Okada S., Mito M., Iwasaki S., Ma D., Zhao X., Asano K., Lin H., Kirino Y., Sakaguchi Y., and Suzuki Ts.:
“Complete chemical structures of human mitochondrial tRNAs”
Nat Commun. 11(1):4269. (2020)
Press Release (RIKEN)
Press Release (The University of Tokyo)
Recommended in Faculty Opinions
Nakazawa K. Shichino Y., Iwasaki S., and Shiina N.:
“Implications of RNG140 (caprin2)-mediated translational regulation in eye lens differentiation”
J Biol Chem. 295(44):15029-15044. (2020)
Press Release (RIKEN)
Press Release (NIBB)
Fujita T., Yokoyama T., Shirouzu M., Taguchi H., Ito T., and Iwasaki S.*:
“Anatomy of footprint extension in ribosome profiling reveals translational landscape mediated by S1 protein in bacteria"
Research Square. (2020)
Saeki N., Eguchi Y., Kintaka R., Makanae K., Shichino Y., Iwasaki S., Kanno M., Kimura N., and Moriya H.:
“N-terminal deletion of Swi3 created by the deletion of a dubious ORF YJL175W mitigates protein burden effect in S. cerevisiae
Sci Rep. 10(1):9500. (2020)
Han P., Shichino Y., Schneider-Poetsch T., Mito M., Hashimoto S., Udagawa T., Kohno K., Yoshida M., Mishima Y., Inada T., and Iwasaki S.*:
“Genome-wide survey of ribosome collision”
Cell Rep. 31(5):107610. (2020)
BioRxiv. (2019)
Press Release
Tsuboyama K., Osaki T., Matsuura-Suzuki E., Kozuka-Hata H., Okada Y., Oyama M., Ikeuchi Y., Iwasaki S., and Tomari Y.:
“A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation”
PLoS Biol. 18(3):e3000632. (2020)
BioRxiv. (2019)
Press Release (The University of Tokyo)
News (Phys.org)
Yokoi S, Naruse K, Kamei Y, Ansai S, Kinoshita M, Mito M, Iwasaki S., Inoue S, Okuyama T, Nakagawa S, Young LJ, and Takeuchi H.:
“Sexually dimorphic role of oxytocin in medaka mate choice”
Proc Natl Acad Sci USA. 117(9):4802-4808. (2020)
Kurihara Y., Makita Y., Shimohira H., Fujita T., Iwasaki S., and Matsui M.:
“Translational landscape of protein-coding and non-protein-coding RNAs upon light exposure in Arabidopsis
Plant Cell Physiol. 61(3):536-545. (2020)
Press Release
Hia F., Yang SF., Shichino Y., Yoshinaga M., Murakawa Y., Vandenbon A., Fukao A., Fujiwara T., Landthaler M., Natsume N., Adachi S., Iwasaki S., and Takeuchi O.:
“Codon bias confers stability to human mRNAs”
EMBO Rep. 20(11):e48220. (2019)
BioRxiv. (2019)
Padrón A., Iwasaki S., and Ingolia NT.:
“Proximity RNA labeling by APEX-Seq reveals the organization of translation initiation complexes and repressive RNA granules”
Mol Cell. 75(4):875-887. (2019)
BioRxiv. (2018)
Hirayama H., Matsuda T., Tsuchiya Y., Oka R., Seino J., Huang C., Nakajima K., Noda Y., Shichino Y., Iwasaki S., and Suzuki T.:
“Free glycans derived from O-mannosylated glycoproteins suggest the presence of an O-glycoprotein degradation pathway in yeast”
J Biol Chem. 294(44):15900-15911. (2019)
Selected as "Editors' Picks Highlights"
Press Release
Fujita T., Kurihara Y., and Iwasaki S.*:
“The plant translatome surveyed by ribosome profiling”
Plant Cell Physiol. 60(9):1917-1926. (2019)
Selected as "Editor's choice"
Mito M., Kadota M., Nakagawa S., and Iwasaki S.*:
“tChIP-Seq: cell-type-specific Eepigenome profiling”
J Vis Exp. 143:e58298. (2019)
Iwasaki S.*, Iwasaki W., Takahashi M. (equal contribution), Sakamoto A., Watanabe C., Shichino Y., Floor SN., Fujiwara K., Mito M., Dodo K., Sodeoka M., Imataka H., Honma T., Fukuzawa K., Ito T., and Ingolia NT.:
“The translation inhibitor rocaglamide targets a bimolecular cavity between eIF4A and polypurine RNA”
Mol Cell. 73(4):738-748.e9 (2019)
Press Release
Recommended in Faculty Opinions
Akichika S., Hirano S. (equal contribution), Shichino Y., Suzuki T., Nishimasu H., Ishitani R., Sugita A., Hirose Y., Iwasaki S., Nureki O., and Suzuki T.:
“Cap-specific terminal N6-methylation of RNA by an RNA polymerase II–associated methyltransferase”
Science. 363(6423):eaav0080. (2019)
Press Release (RIKEN)
Press Release (The University of Tokyo)
Press Release (Nikkei)
Recommended in Faculty Opinions
Komatsu T., Yokoi S., Mito M., Fujii K., Kimura Y., Iwasaki S., and Nakagawa S.:
“UPA-Seq: Prediction of functional lncRNAs using differential sensitivity to UV crosslinking”
RNA. 24(12):1785-1802. (2018)
BioRxiv (2018)
Kurihara Y., Makita Y., Kawashima M., Fujita T., Iwasaki S., and Matsui M.:
“Transcripts from downstream alternative transcription start sites evade uORF-mediated inhibition of gene expression in Arabidopsis
Proc Natl Acad Sci USA. 115(30):7831-7836 (2018)
Highlighted by COMMENTARY
Press Release
Recommended in Faculty Opinions
Mito M., Kadota M., Tanaka K., Furuta Y., Abe K., Iwasaki S.*, and Nakagawa S.:
“Cell-type specific survey of epigenetic modifications by tandem chromatin immunoprecipitation sequencing”
Sci Rep. 8:1143. (2018)
BioRxiv. (2017)
Press Release
Iwasaki S. and Tomari Y.:
“Reconstitution of RNA interference machinery”
Methods Mol Biol.1680:131-143. (2018)
Naruse K., Matsuura-Suzuki E. (equal contribution), Watanabe M., Iwasaki S.*, and Tomari Y.:
“In vitro reconstitution of chaperone-mediated human RISC assembly”
RNA. 24(1):6-11. (2018)
Matsuo Y., Ikeuchi K. (equal contribution), Saeki, Y., Iwasaki S., Schmidt C., Udagawa T., Sato F., Tsuchiya H., Becker T., Tanaka K., Ingolia NT., Beckmann R., and Inada T.:
“Ubiquitination of stalled ribosome triggers ribosome-associated quality control”
Nat Commun. 8(1):159. (2017)
Iwasaki S.* and Ingolia NT.:
“The Growing toolbox for protein synthesis studies”
Trends Biochem Sci. 42(8):612-624. (2017)
Ishikawa K., Makanae K., Iwasaki S., Ingolia NT., and Moriya H.:
“Post-translational dosage compensation buffers genetic perturbations to stoichiometry of protein complexes”
PLoS Genet. 13(1):e1006554. (2017)
Press Release (Okayama University)
News (QLifePro)
Iwasaki S. and Ingolia NT.:
“PROTEIN TRANSLATION. Seeing translation”
Science. 352(6292):1391-2. (2016)
Iwasaki S., Floor SN., and Ingolia NT.:
“Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor”
Nature. 534(7608):558-61. (2016)
Werner A., Iwasaki S., Teerikorpi N., Fedrigo I., Ingolia NT., and Rape M.:
“Cell-fate determination by ubiquitin-dependent regulation of translation”
Nature. 525(7570):523-7. (2015)
Iwasaki S., Sasaki MH. (equal contribution), Sakaguchi Y., Suzuki T., Tadakuma H., and Tomari Y.:
“Defining fundamental steps in the assembly of Drosophila RNAi enzyme complex”
Nature. 521(7553):533-6. (2015)
Press Release
Kwak PB., Iwasaki S. (equal contribution), and Tomari Y.:
“The microRNA pathway and cancer”
Cancer Sci. 101(11), 2309-15 (2010)
Iwasaki S., Kobayashi M. (equal contribution), Yoda M., Sakaguchi Y., Katsuma S., Suzuki T., and Tomari Y.:
“Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes”
Mol Cell. 39(2):292-9. (2010)
Recommended in Faculty Opinions
Yoda M., Kawamata T., Paroo Z., Ye X., Iwasaki S., Liu Q., and Tomari Y.:
“ATP-dependent human RISC assembly pathways”
Nat Struct Mol Biol. 17(1):17-23. (2010)
Iwasaki S., and Tomari Y.:
“Argonaute-mediated translational repression (and activation)”
Fly (Austin). 3(3):204-6. (2009)
Iwasaki S., Kawamata T., and Tomari Y.:
Drosophila Argonaute1 and Argonaute2 employ distinct mechanisms for translational repression”
Mol Cell. 34(1):58-67. (2009)
Journal Cover
Recommended in Faculty Opinions
Takeda A., Iwasaki S., Watanabe T., Utsumi M., and Watanabe Y.:
“The mechanism selecting the guide strand from small RNA duplexes is different among Argonaute proteins”
Plant Cell Physiol. 49(4):493-500. (2008)
Iwasaki S., Takeda A., Motose H., and Watanabe Y.:
“Characterization of Arabidopsis decapping proteins AtDCP1 and AtDCP2, which are essential for post-embryonic development”
FEBS Lett. 581(13):2455-9. (2007)
Japanese Reviews: 2020-2022
市原知哉, 岩崎信太郎, 松本有樹修:
"未注釈ORFの同定とその生理機能"
実験医学 40(12):2003-2010. (2022)
岩崎信太郎:
“ダイソームプロファイリング法によるリボソーム衝突の網羅探索”
生化学 94(1):87-91. (2022)
七野悠一, 岩崎信太郎:
“RNP顆粒研究を加速するトランスクリプトーム解析技術”
相分離生物学の全貌 (東京化学同人) 368-372. (2020)

Japanese Reviews: 2017-2019
木村悠介, 岩崎信太郎:
“リボソームプロファイリングによる網羅的翻訳解析の最前線”
実験医学 37(18):3055‐3057. (2019)
岩崎信太郎:
“eIF4A阻害の多様なメカニズム”
生化学 90(2):211‐215. (2018)
七野悠一, 岩崎信太郎:
“リボソームプロファイリングが切り拓く翻訳研究の未来”
実験医学 35(11):1868‐1873. (2017)

Japanese Reviews: 2010-2016
岩崎信太郎:
“小分子rocaglateは翻訳開始因子eIF4AをRNA配列に対し選択的な翻訳抑制因子に変える”
新着論文レビュー (2016)
岩崎信太郎:
“ncRNA判定法 (リボソームプロファイリング)”
ノンコーディングRNA RNA分子の全体像を俯瞰する (化学同人) 181-191. (2016)
岩崎信太郎、佐々木 浩、泊 幸秀:
“再構成系および1分子観察により同定されたショウジョウバエにおけるRISCの形成の過程”
新着論文レビュー (2015)
岩崎信太郎、泊 幸秀:
“small RNA二本鎖のATP依存的なRISC積み込み反応はHsc70/Hsp90シャペロンマシナリーによって媒介される”
新着論文レビュー (2010)

GO UP